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Int J Mol Cell Med 2012, 1(1): 11-20 Back to browse issues page
Induction of secondary axis in hydra revisited: New insights into pattern formation
Vishal Kadu 1, Saroj S Ghaskadbi 2, Surendra Ghaskadbi *3
1- Division of Animal Sciences, Agharkar Research Institute, Pune, India.
2- Department of Zoology, University of Pune, Pune, India.
3- Division of Animal Sciences, Agharkar Research Institute, Pune, India. , ghaskadbi@gmail.com
Abstract:   (12572 Views)

In 1909, several years before the famous `Organizer’ experiments of Spemann and Mangold, Ethel Browne demonstrated induction of a secondary axis in hydra by grafting a hypostome. Based on this and subsequent work, in the late sixties, Lewis Wolpert proposed the theory of morphogen gradients and positional information. We have studied secondary axis induction by hypostome and foot tissue using three species of hydra as well as transgenic, GFP-expressing lines of hydra. We have found that pieces of hypostome and complete foot of a donor hydra can induce a secondary axis all along (in upper, middle or lower parts of) the body column of a host hydra, both within and across species with comparable rates. Thus, contrary to the available literature, our results show that the host hypostome does not completely inhibit the induction of a secondary axis. The length of the induced axis though is determined by the position of the graft. By using GFP-expressing lines of hydra we have demonstrated that host ectodermal and endodermal cells actively contribute to the secondary axis. On comparison, the hypostome was found to be a stronger and dominant Organizer than the foot. Foot grafting experiments show a transient increase in the host length as well as the distance between the two Organizers. The length becomes normal once the grafted foot reaches the budding zone. Our work brings out several new aspects of the role of positional cues in pattern formation in hydra that can be now be explored at cellular and molecular levels.

Keywords: Hydra, interspecific grafting, morphogen gradients, organizer, induction of secondary axis
Full-Text [PDF 300 kb]   (1840 Downloads)    
Type of Study: Original Article | Subject: Cell Biology
Received: 2011/12/27 | Accepted: 2013/09/14 | Published: 2013/09/14
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Kadu V, Ghaskadbi S S, Ghaskadbi S. Induction of secondary axis in hydra revisited: New insights into pattern formation. Int J Mol Cell Med. 2012; 1 (1) :11-20
URL: http://ijmcmed.org/article-1-25-en.html
Volume 1, Issue 1 (Int J Mol Cell Med 2012) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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