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Aberrant Expression of Immune-related MicroRNAs in Pediatric Patients with Asthma
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Hala Gouda Elnady1    , Lobna Sayed Sherif1 , Naglaa Mohamed Kholoussi2 , Mona Ali Azzam3 , Ahmed Rashad Foda1 , Iman Helwa2 , Rania Nabil Sabry1 , Eman Eissa 4, Reham Faisal Fahmy1 |
1- Department of Child Health, Medical Research Division, National Research Center, Egypt.
2- Immunogenetics Department, Human Genetics and Genome Research Division, National Resrearch Center, Egypt.
3- Department of Pediatrics, Faculty of Medicine, Suez Canal University, Egypt; McMaster University, Hamilton, Canada.
4- Immunogenetics Department, Human Genetics and Genome Research Division, National Resrearch Center, Egypt. , emanessa82@yahoo.com |
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Abstract: (3649 Views) |
| MicroRNAs (miRNAs) have been implicated as regulatory molecules that could play a considerable role in the pathogenesis of different diseases including asthma. This work aims at exploring the role of miR-146a and miR-106b in the pathogenesis of asthma and their association with asthma severity, IgE, and inflammatory cytokines in asthmatic children. Thirty asthmatic children and twenty age-matched healthy children aged 4-17 years old were enrolled. Expression of plasma miR-146a and miR-106b was measured using quantitative real-time PCR. Plasma levels of interleukin-5 (IL-5) and interleukin-13 (IL-13) were assessed using ELISA. Lung functions were measured by Spirometry. MiR-146a and miR-106b were significantly over-expressed in asthmatic children compared to healthy children. A significant positive correlation between total IgE and both miR-146a and miR-106b was found while no significant correlation could be detected between these miRNAs and asthma severity in asthmatic children. Plasma levels of IL-5 and IL-13 were non-significantly higher in asthmatic children compared to healthy children, and there was no significant correlation between them and both miR-146a and miR-106b expressions in the asthmatic children. The aberrant expression of immune-related miRNAs (miR-146a and miR-106b) and inflammatory cytokines (IL-5 and IL-13) among asthmatic children suggest their probable role in asthma pathogenesis. |
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| Keywords: Asthma, microRNAs, cytokines, children, IL-5, IL-13 |
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Full-Text [PDF 275 kb]
(1395 Downloads)
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Type of Study: Original Article |
Subject:
Molecular & Cellular Immunology
Received: 2020/09/7 | Accepted: 2021/01/4 | Published: 2020/11/30
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