en Other Other Original Article Original Article <p style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span lang="EN-IN"><span style="line-height:107%">Doxorubicin (DOX) is a well-known widely-used chemotherapeutic agent in cancer patients. Clinical findings have revealed its association with cardiotoxicity in patients subjected to prolonged administration. Chronic cardiotoxicity may appear after long treatment, with a cumulative dosage exceeding 500&ndash;550 mg/m&sup2; being a critical risk factor. Poppy seed oil is rich in antioxidants and polyunsaturated fatty acids demonstrated efficacy in attenuating oxidative stress and modulating inflammatory responses. In this study, we have investigated the cardioprotective potential of poppy seed oil in DOX-induced cardiotoxicity model of Wistar rats. DOX was administered (2.5 mg/kg) on alternate days for 14 days to induce cardiotoxicity in groups 2, 3 and 4 rats. Poppy seed oil in dose of 500 and 1000 mg/kg was administered orally for 14 days to groups 3 and 4 rats. Cardiac injury biochemical markers (CK-MB, LDH and Troponin-I), oxidative stress (MDA, SOD, CAT, GSH, GR and GPx), and inflammatory cytokines (TNF-&alpha;, IL-6) were assessed. Additionally, histopathological assessment of cardiac tissue was performed to exhibit structural alterations. The administration of poppy seed oil significantly improved cardiac biochemical markers, diminished oxidative stress, and restored antioxidant enzyme levels in DOX-treated rats. It also mitigated myocardial damage, as evidenced by decreased lipid peroxidation and reduced levels of pro-inflammatory cytokines. Histopathological analysis exhibited significant reduction in myocardial degeneration and inflammatory cell infiltration in the poppy seed oil-treated groups. These findings suggest that poppy seed oil may be used as adjunct therapy to reduce chemotherapy-induced cardiac damage, thus warranting further clinical investigation.</span></span></span></span></p> Cardiotoxicity, Cytokines, Cardiac damage, Doxorubicin, Poppy seed oil, Oxidative damage 1158 1170 http://ijmcmed.org/browse.php?a_code=A-10-8759-1&slc_lang=en&sid=1 Tarique Anwer manwr@ub.edu.sa 100319475328460037109 100319475328460037109 Yes Department of Public Health, College of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia. Priyanshi Sharma priyanshisharma199724@gmail.com 100319475328460037110 0009-0002-7203-2944 No HIMT College of Pharmacy, Dr. A.P.J Abdul Kalam Technical University (AKTU), Greater Noida; India Ankit Verma vermaankit1947@gmail.com 100319475328460037111 100319475328460037111 No Department of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India. Abdullah Albaqami Asalbaqame@ub.edu.sa 100319475328460037112 0009-0002-1006-3016 No Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia. Mohammad Firoz Alam malam2@jazanu.edu.sa 100319475328460037113 100319475328460037113 No Department of Pharmacology & Toxicology, College of Pharmacy, Jazan University, Jazan, Saudi Arabia. Ali Mohammed Ali Hazazi alihazzazi.2020@gmail.com 100319475328460037114 100319475328460037114 No Department of Pharmacology & Toxicology, College of Pharmacy, Jazan University, Jazan, Saudi Arabia. Nawazish Alam nrahman@jazanu.edu.sa 100319475328460037115 100319475328460037115 No Department of Clinical Pharmacy, Pharmacy Practice Research Unit, College of Pharmacy, Jazan University, Jazan, Saudi Arabia. Dalia Daws Ddous@jazanu.edu.sa 100319475328460037116 100319475328460037116 No Pharmacy Department, Jazan University Hospital, Jazan University, Jazan, Saudi Arabia.