en Biomarkers (diagnosis & treatment) Biomarkers (diagnosis & treatment) Original Article Original Article <p style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="line-height:14.0pt">The objective of the study was to examine differences in miR-132 gene expression, cytokine levels (IL-1&beta; and IL-6), and functional outcomes in patients with knee osteoarthritis following treatment with PRP and HA. This study employed an exploratory, randomized, open-label, parallel-group interventional research design, where 131 participants were initially recruited. <span lang="ZH-CN">The trial was conducted from January 2023 to January 2024, at Bhayangkara Hospital Makassar, the South Sulawesi Regional Police Headquarters, and the HUM-RC Laboratory, Faculty of Medicine, Hasanuddin University. The samples of peripheral blood were collected at baseline and five days after treatment. miR-132 expression RT-PCR, cytokine levels, and clinical outcomes </span>(<span lang="ZH-CN">VAS and WOMAC scores</span>) were evaluated<span lang="ZH-CN">.</span> PRP significantly decreased miR-132 expression (p = 0.000121), whereas HA (p = 0.775) and controls (p = 0.874) showed no significant changes. Between-group analysis revealed a significant difference between the PRP and control groups (p = 0.0105). IL-1&beta; significantly decreased only in the HA group (p < 0.001). Both treatment groups had lower IL-1&beta; than control group on day five (p < 0.0001). Meanwhile, no significant within- or between-group differences were observed for IL-6. PRP (p = 0.006) and HA (p < 0.001) significantly reduced VAS scores, but only PRP improved WOMAC (p = 0.035). However, the functional outcomes did not surpass the minimal clinically important difference (MCID) thresholds. PRP treatment significantly reduced miR-132 expression and improved functional outcomes to a certain extent, while HA appeared to affect IL-1&beta; modulation with minimal functional effects.</span></span></span></p> Hyaluronic acid, platelet-rich plasma, intra-articular injection, knee osteoarthritis, miR-132, cytokines 1345 1355 http://ijmcmed.org/browse.php?a_code=A-10-8491-1&slc_lang=en&sid=1 Hendrian Chaniago hendrian.chaniago@klinikjurnal.com 100319475328460038272 0009-0000-2355-336X Yes Department of Orthopaedic and Traumatology, Bhayangkara Hospital, Makassar, Indonesia. Muhammad Nasrum Massi nasrumm200@yahoo.com 100319475328460038273 0009-0000-4738-6279 No Department of Clinical Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia Muhammad Andry Usman m.andry.usman@gmail.com 100319475328460038274 100319475328460038274 No Department of Orthopaedics, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia Muhammad Phetrus Johan muhpjo@gmail.com 100319475328460038275 100319475328460038275 No Department of Orthopaedics, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia